SARS-CoV-2 Spike Glycoprotein RBD (319-541), South Africa Variant, His tag

Catalog #: EG-323

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Cat #	EG-323
Description	A recombinant form of South African variant of the spike glycoprotein receptor binding domain (RBD, Arg319-Phe541) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2). This RBD mutant has K417N, E484K and N501Y mutations in the S glycoprotein RBD as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). It was produced in HEK293 cells and purified by nickel affinity and ion exchange chromatography. This recombinant protein contains 223 residues of the SARS-CoV-2 spike glycoprotein RBD with a C-terminal hexa-histidine tag.
Mutations	K417N, E484K and N501Y
Tags	C-terminal hexa-histidine
Purification	FPLC by nickel affinity and ion exchange chromatography
Sequence	1 RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL 51 YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGNI 101 ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI 151 STEIYQAGST PCNGVKGFNC YFPLQSYGFQ PTYGVGYQPY RVVVLSFELL 201 HAPATVCGPK KSTNLVKNKC VNFHHHHHH
Mol Wt	25.96 kDa
Source	HEK 293
Purity	90~95% by SDS-PAGE
Applications	Western blot, ELISA and animal vaccination
Storage Buffer	PBS + 50% Glycerol
Storage	-20ºC ~ -80ºC
Shipping	dry ice
References

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