| Product Name |
SARS-CoV-2 Spike Glycoprotein RBD, United Kingdom Variant, His Tag |
| Catalog Number |
EG-324 |
| Description |
Recombinant SARS-CoV-2 spike glycoprotein receptor-binding domain (RBD, Arg319–Phe541, United Kingdom variant, B.1.1.7) with N501Y mutation compared to Wuhan-Hu-1 (GenBank: QHD43416.1). Expressed in HEK293 cells, purified by FPLC using nickel affinity and ion exchange chromatography, with a C-terminal 6xHis tag. |
| Mutations |
N501Y |
| Tags |
C-terminal 6xHis |
| Purification |
FPLC (nickel affinity and ion exchange chromatography) |
| Sequence |
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFK 60
CYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNS 120
NNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQ 180
PTYGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFHHHHHH 228
|
| Molecular Weight |
26.0 kDa (theoretical) |
| Source |
HEK293 cells |
| Purity |
≥90% by SDS-PAGE |
| Applications |
Western blot, ELISA, animal immunization studies |
| Storage Buffer |
PBS with 50% glycerol |
| Storage |
-20°C to -80°C |
| Shipping |
Dry ice |
|