| Product Name |
PolyExpress™ Transfection Reagent |
| Catalog Number |
EG-1072 |
| Size |
1 mL |
| Description |
PolyExpress™ Transfection Reagent, a biodegradable cationic polymer-based formulation, delivers DNA with high transfection efficiency and minimal cytotoxicity to a wide range of mammalian cell lines, including HEK293, COS-7, NIH 3T3, HeLa, CHO, and hard-to-transfect cells, forming stable polyplexes rapidly at room temperature. |
| Advantages |
- Biodegradable, reducing cytotoxicity post-endocytosis
- Supports high-titer viral production
- Effective for large DNA constructs (>89 kb)
- Efficient for single and multi-DNA co-transfections
- Enables high levels of recombinant protein production
- Simple and robust transfection protocol
- Cost-effective
|
| Transfection Efficiency |
-
Figure 1. Comparison of transfection efficiency between PolyExpress™ and Lipofectamine 2000 in HEK293 cells.
-
Figure 2. Transfection of glioma xenograft cell lines (5310, 4910) using GeneExpresso™ and PolyExpress™ (courtesy of Dr. Chandram Chetty, University of Illinois).
-
Figure 3. Comparison of transfection efficiency of PolyExpress™ (#1, #2, #3) with other transfection reagents (#4–#8) in HEK293 cells using 3 µL reagent and 1 µg DNA in a 12-well plate.
|
| Protocol |
DNA Transfection Protocol
|
| Quality Control |
- Functionally validated for high transfection efficiency in HEK293 and CHO cells
- Confirmed low cytotoxicity in standard cell culture conditions
|
| Storage |
Store at 4°C; stable for 12 months or longer if stored properly |
| Shipping |
Ambient temperature |