| Product Name |
GeneExpresso™ Transfection Reagent |
| Catalog Number |
EG-1031 |
| Size |
1 mL |
| Description |
GeneExpresso™ Transfection Reagent, a proprietary cationic polymer-lipid conjugate, delivers DNA with high transfection efficiency and low cytotoxicity to a wide range of eukaryotic cell lines, including HEK293, 293T, 293E, CHO, COS-1, HeLa, NIH 3T3, Sf9, Sf21, and primary cells. |
| Advantages |
- High transfection efficiency for difficult-to-transfect cells
- Compatible with a broad range of eukaryotic cell lines, including mammalian and insect cells
- Low cytotoxicity
- Effective in serum-containing media without interference
- Highly stable formulation
- Simple, user-friendly protocol
- Suitable for high-throughput applications
|
| Transfection Efficiency |
-
Figure 1. FACS analysis comparing transfection efficiency of GeneExpresso™ and Lipofectamine 2000 in HeLa cells.
-
Figure 2. Transfection of glioma xenograft cell lines (5310, 4910) using GeneExpresso™ and PolyExpress (courtesy of Dr. Chandram Chetty, University of Illinois).
-
Figure 3. Transfection efficiency of GeneExpresso™ in HEK293 cells.
-
Figure 4. FACS analysis comparing transfection efficiency of GeneExpresso™, GeneExpresso 8000, and PolyExpress in multiple cell lines.
| Cell Lines |
Efficiency (% GFP) |
Cell Lines |
Efficiency (% GFP) |
3LL
B16-F10
BAEC
BHK-21
Ca Ski
CaCo2
CHO
CLBPEC
COS-7
CV-1
D 407
DHD Pro.b
HCS-2/8
HEK293
HeLa
HLMEC
H-MVEC
Huh-7D12
ATT20
|
50%
73%
51%
82%
70%
31%
81%
10%
70%
51%
61%
53%
41%
85%
80%
60%
50%
23%
39%
|
K562
L929
MCF-7
MDCK
Neuro2A
NIH 3T3
PC12
Primary human keratinocytes
Primary human pre-adipocytes
Primary human skin fibroblasts
SAOS-2
SHEP
SH-SY5Y
SiHa
SKOV3
HUVEC
IGROV1
Jurkat
6CSFMEo
|
25%
54%
53%
54%
71%
80%
31%
25%
25%
31%
51%
70%
15%
63%
53%
52%
22%
5%-15%
79%
|
|
| Protocol |
DNA Transfection Protocol
|
| Quality Control |
- Functionally validated for high transfection efficiency in HEK293 and CHO cells
- Confirmed low cytotoxicity in standard cell culture conditions
|
| Storage |
Store at 4°C; stable for 18 months or longer if stored properly |
| Shipping |
Ambient temperature |
|