| Product Name |
D7324 Tag, Mouse Monoclonal Antibody (Clone 1E2) |
| Overview |
The D7324 tag (APTKAKRRVVQREKR) is derived from the conserved C-terminus of HIV-1 gp120, corresponding to amino acids 497–511 of the BH10 strain (Ratner L. et al., Nature, 313, 277-284, 1985). Initially, this epitope was used for capturing HIV-1 envelope proteins with a goat polyclonal antibody (D7324) in capture ELISA assays. Subsequently, the D7324 tag has been widely adopted as an epitope tag for recombinant soluble membrane proteins—including those from HIV-1, SIV, Dengue, Zika, and Lassa virus—for capture ELISA in antibody response detection and monoclonal antibody screening. Many recombinant viral envelope proteins are engineered to express a C-terminal D7324 tag, which does not interfere with protein bioactivity. The 1E2 monoclonal antibody specifically captures C-terminal D7324-tagged fusion proteins as well as native Env from lysates of HIV-1 virions or infected cells.
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| Clone Number |
1E2 |
| Host |
Mouse |
| Class |
Recombinant Monoclonal |
| Immunogen |
D7324 tag peptide (APTKAKRRVVQREKR) |
| Isotype |
IgG2a, kappa |
| Species Cross-Reactivity |
N/A |
| Purity |
Purified from serum-free HEK293F culture supernatant by Protein A affinity chromatography |
| Concentration |
~1 mg/mL |
| Formulation |
1× DPBS, pH 7.4, without additives |
| Quality Control |
D7324 Tag Antibody QC
50 µL of 4 µg/mL 1E2 mAb in PBS was coated on an ELISA plate overnight at 4 °C. D7324-tagged protein supernatant from transiently transfected HEK293F cells was captured and detected using a protein-specific detection antibody.
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| Shipping |
Shipped on blue ice |
| Storage |
-20 °C (stable for 1 year)
4 °C (short term)
For long-term storage, avoid repeated freeze-thaw cycles by aliquoting.
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