| Description |
GRRed Nucleic Acid Loading Buffer is a 6X gel loading buffer containing GRRed Nucleic Acid Stain—a safe, highly sensitive fluorescent dye for instant visualization of DNA bands during or immediately after agarose gel electrophoresis. The dye forms a tight complex with nucleic acids, comigrates with samples, and enables direct viewing on a standard UV transilluminator. No post-staining or destaining is required. GRRed offers a faster, safer, and more environmentally friendly alternative to ethidium bromide.
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| Key Benefits |
- Safe: Non-mutagenic (Ames test certified); gels can be disposed of as regular trash
- Easy: Supplied as a 6X loading buffer; simply mix with nucleic acid samples, load, run, and visualize
- Sensitive: More sensitive than ethidium bromide and SYBR Safe
- Economical: No hazardous shipping, storage, or waste disposal costs
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| Applications |
- Routine agarose gel electrophoresis of DNA and RNA samples
- Visualization of nucleic acid markers and ladders
- Sample preparation for downstream purification
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| Protocol |
Add 2 µl of 6X GRRed Loading Buffer to 10 µl DNA or RNA sample. Load and run the gel as usual. Visualize bands using a UV transilluminator.
View full protocol (PDF)
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| Storage & Handling |
Store at -20 °C, protected from light. Stable for 1 year at -20 °C and 3 months at 4 °C.
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| Removal |
GRRed dye can be completely removed from nucleic acids by alcohol precipitation or Qiagen QIAquick Gel Extraction.
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| Performance Example |
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