| Product Name |
D7324 Tag, Mouse Monoclonal Antibody 1E2 |
| Overview |
The D7324 tag (APTKAKRRVVQREKR) is derived from the conserved C terminal of HIV-1 gp120 corresponding to amino acids numbers 497-511 of the BH10 strain of HIV-1 (Ratner , L. et al., Nature, 313, 277-284 (1985). The target was first used for capturing HIV-1 envelope with a goat polyclonal antibody D7324 in capture ELISA format. It has then been used as an epitope tag in expression of recombinant soluble membrane proteins (including HIV-1, SIV, Dengue, Zika, and Lassa virus (LASV), et al) for conventional capture ELISA in detection of antibody immune response and monoclonal antibody screening and characterization. Many recombinant viral envelope proteins have been engineered to express C-terminal D7324 tag, which does not appear to interfere with the bioactivity of the recombinant protein. The monoclonal antibody 1E2 can capture C-terminal, D7324-tag fusion proteins as well as many native Env from lysate of HIV-1 virions or infected cells |
| Clone Number |
1E2 |
| Host |
Mouse |
| Class |
Recombinant Monoclonal |
| Immunogen |
D7324 tag (APTKAKRRVVQREKR) |
| Isotype |
IgG2a/kappa |
| Species Cross-Reactivity |
N/A |
| Purity |
The antibody was purified from serum-free 293F culture supernatant by Protein A affinity-chromatography |
| Concentration |
~1 mg/ml |
| Formulation |
1 x DPBS, pH 7.4, without additive |
| QC |
50 µl of 4 µg/ml 1E2 mAb in PBS was coated in ELISA plate overnight at 4 oC. D7324-tagged protein supernatant from transient transfection of 293F cells was then captured and detected by a protein specific detection antibody.
|
| Shipping |
Blue ice |
| Storage |
-20 oC or 4 oC. Stable for 1 year at -20 oC |