| Product Name |
Anti-B7-H3 monoclonal
antibody, recombinant |
| Cat # |
EG-372 |
| Size |
100 µg |
| Key features |
Rabbit monoclonal antibody
produced in 293 cells with serum-free, protein-free medium, purified
with protein A column;
High batch-to-batch consistency and reproducibility;
Long term security of supply; |
| Clone number |
C10 |
| Immunogen |
Recombinant extracellular
domain of B7-H3 that was expressed and purified from 293 cells |
| Reaction
target |
Extracellular region of
B7-H3 |
| Species
Cross-Reactivity |
Human |
| Applications |
Flow Cytometry; IP; ELISA; |
| Working
concentrations |
Flow cytometry and ELISA: 5
µg/ml of final concentration is suggested.
IP: 10 µg in 0.3-0.5 mg cell lysates is suggested.
|
| Background |
B7-H3 (also known as CD276)
is a member of B7 family, which is over-expressed in many cancer types.
B7-H3 is an immune checkpoint protein that inhibits tumor
antigen-specific immune responses. B7-H3 can also promote tumor
migration, invasion, angiogenesis, chemoresistance,
epithelial-to-mesenchymal transition (EMT), and regulate tumor cell
metabolism (Kontos F, et al. B7-H3: an attractive target for
antibody-based immunotherapy. Clinical Cancer Research. 2020, 27:
1227–1235). B7-H3 is regarded as an attractive target for cancer
immunotherapy. Several anticancer agents are targeting B7-H3, such as
enoblituzumab (MGA271), omburtamab, MGD009, MGC018, DS-7300a, and CAR T
cells. |
| QC |
Flow cytometry assay showing cell surface expression of
B7-H3. KLM-1 cells (10^6) were incubated with 5 µg antibody in 1 ml PBS
buffer containing 5% BSA. Antibody binding was detected with
Cy5-conjugated goat-anti-rabbit antibody. Blue line, isotype control
antibody staining; red line, B7-H3 antibody staining.
|
| Concentration |
As indicated on the vials |
| Formulation |
80% PBS, pH 7.4; 20%
Glycerol; 5% Trehalose; 0.04% Sodium azide as Preservative |
| Shipping |
Blue ice |
| Storage |
-20 to -70 oC
(long term)
or 4 oC (one week).
Depending on the expected frequency of use, the product can be
aliquoted to avoid heavily repeated freezing/thawing cycles. |