| Service Name |
2 L Expression and FPLC Purification Service |
| Description |
Our 2 L Expression and FPLC Purification Service provides high-yield recombinant protein production and purification from a 2-liter culture scale. This comprehensive service includes small-scale test expression, large-scale expression in your preferred host (typically E. coli), and purification using state-of-the-art ÄKTA FPLC (Fast Protein Liquid Chromatography) systems. The workflow is optimized for maximum protein yield, purity, and activity, and is suitable for research, assay development, and structural biology applications.
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| Key Features |
- 2-liter culture scale for robust protein yields
- Customizable expression in E. coli or other hosts (inquire for yeast, insect, or mammalian options)
- Purification via affinity, ion exchange, size exclusion, or multi-step FPLC workflows
- Compatible with a wide range of affinity tags (His, GST, MBP, Strep, etc.)
- Optional tag removal and polishing steps
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| Workflow |
- Small-scale expression testing and optimization
- Large-scale (2 L) culture expression
- Cell harvest and lysis
- FPLC purification (affinity, ion exchange, size exclusion, or combination)
- Buffer exchange and concentration (if required)
- Comprehensive quality control
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| Quality Control |
- SDS-PAGE and Coomassie staining (>95% purity)
- Western blot analysis (if antibody is available)
- Protein concentration determination
- Chromatograms and process documentation
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| Deliverables |
- Pooled, purified protein in your specified buffer and concentration
- Quality control report (SDS-PAGE, purity, yield, activity, etc.)
- Chromatograms and method summary
- Optional: expression plasmid, cell pellets, or lysate upon request
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| Sample Requirements |
- Expression plasmid or gene sequence
- Preferred tag and purification method (if applicable)
- Any special buffer or storage requirements
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| Applications |
- Structural biology (X-ray, NMR, cryo-EM)
- Enzyme assays and functional studies
- Antibody production
- Drug screening and target validation
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| Consultation & Support |
Free project consultation and technical support throughout your project.
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| Contact |
Contact us for a custom quote and to discuss your specific project requirements.
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