| Product Name |
NLS-Cre Recombinase (NLS-Cre) mRNA |
| Catalog Number |
EG-2589 |
| Description |
NLS-Cre mRNA, synthesized from animal-free components, expresses a human- and mouse-codon-optimized Cre recombinase fused to a nuclear localization sequence (NLS), enabling efficient ex vivo loxP recombination in cell lines and primary cells. |
| Codon Optimization |
Human and mouse |
| Modifications |
- 5’ capped with ARCA (m7(3’-O-methyl)-G(5’)ppp(5’)G, Cap 0) or CleanCap AG (m7G(5’)ppp(5’)(2’-OMeA)pG, Cap 1)
- Pseudouridine-5’-triphosphate (Pseudo-UTP)
- 5-Methylcytidine-5’-triphosphate (5mCTP)
- Poly-A tail: 180 nucleotides
|
| Applications |
- Ex vivo loxP recombination in cell lines and primary cells
|
| Concentration |
~1 µg/µL |
| Transfection Protocol |
Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
|
| Quality Control |
- Integrity verified by agarose gel electrophoresis
- Functional activity confirmed by transfection of HEK293 cells with a loxP reporter plasmid
|
| Storage |
-80°C |
| Shipping |
Dry ice |